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Analysis of serotonin in brain microdialysates using capillary electrophoresis and native laser-induced fluorescence detection

✍ Scribed by Nadia Benturquia; François Couderc; Valérie Sauvinet; Cyrille Orset; Sandrine Parrot; Christophe Bayle; Bernard Renaud; Luc Denoroy

Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
166 KB
Volume
26
Category
Article
ISSN
0173-0835

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✦ Synopsis

Analysis of serotonin in brain microdialysates using capillary electrophoresis and native laser-induced fluorescence detection

Serotonin or 5-hydroxytryptamine (5-HT) is a major neurotransmitter in the central nervous system. In this work, a method for analyzing 5-HT in brain microdialysis samples using a commercially available capillary electrophoresis (CE) system has been developed. A pH-mediated in-capillary preconcentration of samples was performed, and after separation by capillary zone electrophoresis, native fluorescence of 5-HTwas detected by a 266 nm solid-state laser. The separation conditions for the analysis of 5-HT in standard solutions and microdialysates have been optimized, and this method has been validated on both pharmacological and analytical bases. Separation of 5-HT was performed using a 80 mmol/L citrate buffer, pH 2.5, containing 20 mmol/L hydroxypropyl-b-cyclodextrin (HP-b-CD) and 130 kV voltage. The detection limit was 2.5610 210 mol/L. This method allows the in vivo brain monitoring of 5-HT using a simple, accurate CE measurement in underivatized microdialysis samples.

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