Postcolumn derivatization of proteins in capillary sieving electrophoresis/laser-induced fluorescence detection
β Scribed by Takashi Kaneta; Daisuke Yamamoto; Totaro Imasaka
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 178 KB
- Volume
- 30
- Category
- Article
- ISSN
- 0173-0835
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Aequorea victoria green fluorescent protein was assayed by capillary electrophoresis using post-capillary laserinduced fluorescence detection in a sheath flow cuvette. The limit of detection was 3.0 Ο« 10 Οͺ 12 M protein in an injection volume of 17 nL, corresponding to a mass of 3100 molecules.