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Analysis of proteins in biological samples by capillary sieving electrophoresis with postcolumn derivatization/laser-induced fluorescence detection

✍ Scribed by Takashi Kaneta; Takehito Ogura; Totaro Imasaka

Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
213 KB
Volume
32
Category
Article
ISSN
0173-0835

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## Abstract Carbonyl‐modified proteins are markers of oxidative damage. Here, we report a new method for detecting and quantifying carbonylated proteins by capillary sieving electrophoresis (CSE) with LIF detection (CSE‐LIF). Alexa 488 hydrazide is used for the specific labeling of carbonyls while