Laser-induced fluorescence detection schemes for the analysis of proteins and peptides using capillary electrophoresis

✍ Scribed by Marlene Lacroix; Véréna Poinsot; Clara Fournier; François Couderc

• Publisher: John Wiley and Sons
• Year: 2005
• Tongue: English
• Weight: 491 KB
• Volume: 26
• Category: Article
• ISSN: 0173-0835
• DOI: 10.1002/elps.200410414

✦ Synopsis

Abstract

Over the past few years, a large number of studies have been prepared that describe the analysis of peptides and proteins using capillary electrophoresis (CE) and laser‐induced fluorescence (LIF). These studies have focused on two general goals: (i) development of automatic, selective and quick separation and detection of mixtures of peptides or proteins; (ii) generation of new methods of quantitation for very low concentrations (nm and subnanomolar) of peptides. These two goals are attained with the use of covalent labelling reactions using a variety of dyes that can be readily excited by the radiation from a commonly available laser or via the use of noncovalent labelling (immunoassay using a labelled antibody or antigen or noncovalent dye interactions). In this review article, we summarize the works which were performed for protein and peptide analysis via CE‐LIF.