✦ LIBER ✦

Analysis of leukotrienes by high-pressure liquid chromatography

✍ Scribed by W.Rodney Mathews; Joshua Rokach; Robert C. Murphy

Publisher: Elsevier Science
Year: 1981
Tongue: English
Weight: 527 KB
Volume: 118
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(81)90162-7

No coin nor oath required. For personal study only.

✦ Synopsis

A reversed-phase high-pressure liquid chromatographic procedure has been developed to separate and quantitate leukotrienes. This procedure is based on the ultraviolet absorption of the conjugated triene moiety of these compounds and is sensitive to the nanogram level. Several leukotrienes (B4, C,, D4) can be quantitated in a singie analysis using prostaglandin Bz as an internal standard. Leukotriene production by murine m~t~ytoma cells foliowing stimulation with a calcium ionophore has been examined by this method.

📜 SIMILAR VOLUMES

Analysis of Leukotrienes, Lipoxins, and

Analysis of Leukotrienes, Lipoxins, and Monooxygenated Metabolites of Arachidonic Acid by Reversed-Phase High-Pressure Liquid Chromatography

✍ W.G. Yu; W.S. Powell 📂 Article 📅 1995 🏛 Elsevier Science 🌐 English ⚖ 759 KB

Analysis of trisulfapyrimidines by high-

Analysis of trisulfapyrimidines by high-pressure liquid chromatography

✍ Raymond B. Poet; Henry H. Pu 📂 Article 📅 1973 🏛 John Wiley and Sons 🌐 English ⚖ 324 KB 👁 1 views

A To whom inquiries should be directed.

Analysis of metallonucleoside hydrolysis

Analysis of metallonucleoside hydrolysis by high-pressure liquid chromatography

✍ Michael J. Clarke; Katherine F. Coffey; Holly J. Perpall; John Lyon 📂 Article 📅 1982 🏛 Elsevier Science 🌐 English ⚖ 628 KB

Analysis of enzymatic steroid conversion

Analysis of enzymatic steroid conversions by high pressure liquid chromatography

✍ S.A. Slocum; J.F. Studebaker 📂 Article 📅 1975 🏛 Elsevier Science 🌐 English ⚖ 271 KB

Enzyme catalyzed introduction of the 1-2 double bond into a steroid can be monitored through spectrophotometric changes accompanying electron acceptor reduction or through paper or thin-layer chromatographic analysis of the reaction product. The spectrophotometric method is not applicable to cases i

Analysis of sugars in glycoproteins by h

Analysis of sugars in glycoproteins by high-pressure liquid chromatography

✍ Neil Jentoft 📂 Article 📅 1985 🏛 Elsevier Science 🌐 English ⚖ 802 KB

A method for analyzing the carbohydrate composition of glycoproteins and similar glycoconjugates by methanolysis followed by reverse-phase high-pressure liquid chromatography of the perbenzoylated methyl glycosides has been developed. As described, the method is capable of quantifying sugars in the

Analysis of cardenolides of Convallaria

Analysis of cardenolides of Convallaria majalis by high pressure liquid chromatography

✍ Harkiss, K. J. ;Linley, P. A. ;Mesbah, M. K. M. 📂 Article 📅 1981 🏛 John Wiley and Sons 🌐 English ⚖ 174 KB 👁 1 views

## Abstract Cardenolides of __Convallaria majalis__ were separated by reverse‐phase HPLC on Partisil PXS 10/25 ODS columns using 25% acetonitrile as mobile phase. Periplorhamnoside, convallatoxin and either convallatoxol or convalloside were resolved from glycoside mixtures. Convallatoxin and perip