Analysis of sugars in glycoproteins by high-pressure liquid chromatography

✍ Scribed by Neil Jentoft

• Publisher: Elsevier Science
• Year: 1985
• Tongue: English
• Weight: 802 KB
• Volume: 148
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(85)90248-9

✦ Synopsis

A method for analyzing the carbohydrate composition of glycoproteins and similar glycoconjugates by methanolysis followed by reverse-phase high-pressure liquid chromatography of the perbenzoylated methyl glycosides has been developed. As described, the method is capable of quantifying sugars in the I-to IO-nmol range while further optimization of procedures may increase the usable sensitivity by a factor of 10 or greater. Improved yields of the sugar derivatives have been achieved by incorporating several modifications of the original methanolysis procedure. This, together with the use of high-pressure liquid chromatography rather than gas chromatography for separating the sugar derivatives, eliminates the need for empirically determined molar response ratios. 0 1985 Academic PRSS, h.