✦ LIBER ✦

Sugar analysis of glycoproteins and glycolipids after methanolysis by high-performance liquid chromatography with pulsed amperometric detection

✍ Scribed by Anja Lampio; Jukka Finne

Publisher: Elsevier Science
Year: 1991
Tongue: English
Weight: 481 KB
Volume: 197
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(91)90368-4

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✦ Synopsis

A procedure for the analysis of the monosaccharide composition of glycoproteins and glycolipids by methanolysis and high-performance liquid chromatography with pulsed amperometric detection is described. The advantage over previous methods is the analysis of underivatized methyl glycosides of all glycoconjugate monosaccharides including sialic acid and uronic acid in a single chromatographic step at the subnanomolar level.

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