A procedure for the analysis of the monosaccharide composition of glycoproteins and glycolipids by methanolysis and high-performance liquid chromatography with pulsed amperometric detection is described. The advantage over previous methods is the analysis of underivatized methyl glycosides of all gl
Microdialysis sampling and high performance liquid chromatography with amperometric detection for sugar analysis in milk products
✍ Scribed by Saverio Mannino; Maria S. Cosio; Paola Zimei
- Publisher
- John Wiley and Sons
- Year
- 1996
- Tongue
- English
- Weight
- 264 KB
- Volume
- 8
- Category
- Article
- ISSN
- 1040-0397
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✦ Synopsis
Abstract
A rapid and highly efficient method for the direct quantification of the major carbohydrates, glucose, fructose, sucrose and lactose in milk‐based products is described. Rapid and selective monitoring of each sugar is accomplished by microdialysis sampling coupled with HPLC separation and amperometric detection with a chemically modified electrode. This new scheme offers simultaneous monitoring of sugars with little or no sample pretreatment. The results obtained from the application of the proposed procedure to milk and milk‐based products are discussed.
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