HepG2 lysates. As expected, the highest signal was found for the liver cell line (24). The proposed assay is highly sensitive and allows screening of high numbers of samples in an easy and rapid way. All these characteristics together make the Determination of Cysteine Residues in Protein test very
Analysis of inorganic pyrophosphate at the picomole level
โ Scribed by Chi P. Cheung; Robert J. Suhadolnik
- Publisher
- Elsevier Science
- Year
- 1977
- Tongue
- English
- Weight
- 162 KB
- Volume
- 83
- Category
- Article
- ISSN
- 0003-2697
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A new method for the rapid analysis of inorganic pyrophosphate (PPJ which utilizes the enzyme ATP sulfurylase is described. All components of the assay system are commercially available and inexpensive. The assay is linear over the range of 0.5-50.0 nmol of PP, and is not affected by inorganic phosp
A new sensitive method is described for glucose l-phosphate analysis. The key reaction is the pyrophosphorolysis of UDP-glucose catalyzed by uridine Y-diphosphoglucose pyrophosphorylase. The reaction product, ['4C]UDP-giucose, is separated from [W]UTP by adsorbing [W]UTP selectively onto polyethylen