Analysis of NAD+ in picomole amounts with sodium [32P]pyrophosphate and NAD-pyrophosphorylase

Cleavage at acyl-proline bonds with sodi

Cleavage at acyl-proline bonds with sodium in liquid ammonia: Application with nanomolar amounts of peptides and separation of products byhigh-performance liquid chromatography for structural analysis

✍ John Hempel; Hans Jörnvall 📂 Article 📅 1985 🏛 Elsevier Science 🌐 English ⚖ 998 KB

Cleavage of X-Pro bonds with metallic sodium in liquid ammonia is a little-used method due to difficulties with handling of reagents, variable cleavage yields, and separation of peptides from salt byproducts. Construction of a small distillation/reaction apparatus permitted peptide incubations at th