A new sensitive method is described for glucose l-phosphate analysis. The key reaction is the pyrophosphorolysis of UDP-glucose catalyzed by uridine Y-diphosphoglucose pyrophosphorylase. The reaction product, ['4C]UDP-giucose, is separated from [W]UTP by adsorbing [W]UTP selectively onto polyethylen
Analysis of adenine nucleotides at the picomole level with 32P-phosphoenolpyruvate and pyruvate kinase
โ Scribed by Chi P. Cheung; Abraham Marcus
- Publisher
- Elsevier Science
- Year
- 1975
- Tongue
- English
- Weight
- 477 KB
- Volume
- 69
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
The functional importance of glycolipids has emphasized the need for more sensitive methods of detection, characterization, and quantification than has often been possible using traditional thin-layer chromatographic techniques. We describe the use of ceramide glycanase and HPLC to identify and quan
A method for quantitative determination of biological thiols is presented. Thiols are converted to fluorescent derivatives by reaction with monobromobimane or monobromotrimethylammoniobimane. The derivatives are separated by ion-exchange chromatography and detected by fluorometry. Thiols that can be