Analysis of CD4+CD25+regulatory T cells and Foxp3 mRNA in the peripheral blood of patients with asthma
β Scribed by Keying Xue; Yongming Zhou; Shengdao Xiong; Weining Xiong; Tao Tang
- Publisher
- Springer-Verlag
- Year
- 2007
- Tongue
- Chinese
- Weight
- 71 KB
- Volume
- 27
- Category
- Article
- ISSN
- 0510-9752
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The transcription factor FOXP3 plays a key role in CD4 + CD25 + regulatory T cell function and represents a specific marker for these cells. Despite its strong association with regulatory T cell function, in humans little is known about the frequency of CD4 + CD25 + cells that express FOXP3 protein
the authors want to correct the Methods section regarding the fixation/permeabilization protocols used for this study. The authors mentioned the use of 4% paraformaldehyde (PFA) on pages 2 and 3 to resuspend the cells lasting the final step before flow acquisition, when in reality 1% PFA was used. F