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Analysis of a method for selecting temperature-sensitive mutants of BHK cells

✍ Scribed by Takeharu Nishimoto; Claudio Basilico

Book ID
112559336
Publisher
Springer
Year
1978
Tongue
English
Weight
805 KB
Volume
4
Category
Article
ISSN
1572-9931

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πŸ“œ SIMILAR VOLUMES

A temperature-sensitive cell cycle mutan
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## Abstract A temperature‐sensitive growth mutant derived from the BHK 21 cell Line, ts AF8, was found to have greatly reduced DNA synthesis at the nonpermissive temperature. This reduction is mainly due to a decrease in the frequency of cells synthesizing DNA. Upon shift up, ts AF8 becomes blocked

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## Abstract Three different temperature sensitive mutants derived from the Syrian hamster cell line BHK 21 were found to have greatly reduced DNA synthesis at the non‐permissive temperature. These mutants are distinct by complementation analysis and behave at the non‐permissive temperature as cell