Ribosome metabolism in temperature-sensitive mutant of BHK cells
✍ Scribed by KUMAR, A.; BANDMAN, E.; MELVIN, W. T.
- Book ID
- 109698377
- Publisher
- Nature Publishing Group
- Year
- 1976
- Tongue
- English
- Weight
- 323 KB
- Volume
- 259
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/259692a0
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## Abstract Three different temperature sensitive mutants derived from the Syrian hamster cell line BHK 21 were found to have greatly reduced DNA synthesis at the non‐permissive temperature. These mutants are distinct by complementation analysis and behave at the non‐permissive temperature as cell
## Abstract A mutant of BHK cells (ts422E) temperature‐sensitive for processing 32S rRNA to 28S rRNA (Toniolo et al., '73) also loses the ability to synthesize polyamines and 5.8S rRNA when shifted to the non‐permissive temperature (39°). The activity of several enzymes not involved with polyamine
## Abstract A temperature‐sensitive growth mutant derived from the BHK 21 cell Line, ts AF8, was found to have greatly reduced DNA synthesis at the nonpermissive temperature. This reduction is mainly due to a decrease in the frequency of cells synthesizing DNA. Upon shift up, ts AF8 becomes blocked