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An optimized polystyrene support for rapid, efficient oligonucleotide synthesis

✍ Scribed by Christie McCollum; Alex Andrus

Publisher: Elsevier Science
Year: 1991
Tongue: French
Weight: 283 KB
Volume: 32
Category: Article
ISSN: 0040-4039
DOI: 10.1016/s0040-4039(00)79865-0

No coin nor oath required. For personal study only.

✦ Synopsis

An opnmlzed t);ae ofpolystyrene has been developed us n solid slipportfor automated oligonucleotlde synthesis The non-swellulg, rlgld beads ~OJJ~JJ the (lttr(xnve features of rapid reu[ non kmetxj, ej~c~lcrenf washmg with orgaruc solvents, and rnecharucal stnbrllv Pore ~lze, part& size, and pnlymerizatmn formula and cnndzinns have been optlmlzed The support muterud 1s derlvatlzed to give primary amlnnfunctionallty, which IS near quantuatlveiy coupled to 3'pmrrophenyi .sllccmute nucleosldes Loading of the nucleosrdes can be preclseiy controlled III a runge ol.5 to 70 woie per grcztn The high yield syntheslr of ohgonucleoades, by phosphommidlte chemistry has been demonstrated 11p to 120 ~~PCC In length Stdc reactlonl, such as extraneous chum growth, ure mlmmlzed due to the lack of recur tl1.e fum tronnliv on the new rllpport sulrface.

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