A direct and efficient method for derivatisation of solid supports for oligonucleotide synthesis
β Scribed by Andrew J. Walsh; G.Claudine Clark; William Fraser
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- French
- Weight
- 235 KB
- Volume
- 38
- Category
- Article
- ISSN
- 0040-4039
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π SIMILAR VOLUMES
## Abstract Oligonucleotides containing terminal phosphate groups serve as useful intermediates for different applications in molecular and cell biology, as well as for diagnostic purposes. The chemical synthesis of these derivatives has been an important topic of oligonucleotide research. We repor
Michaelis-Arbusov chemistry was used to prepare O,S-dialkyl Y-O-nucleosidyl phosphorothiolate triesters in solution and attached to CPG. The support-bound nucleoside was utilised in the synthesis of a pentaribonucleotide that was fully deprotected on the support. Subsequent ~'eatment with a buffere