In the present study, we describe a method that we developed to isolate total RNA from porcine adipose tissue. This method entails homogenizing porcine adipose tissue in 10 ml of 4 M guanidium thiocyanate, 25 mM sodium citrate, 0.5% Sarcosyl, 0.1 M beta-mercaptoethanol, pH 7.0, and then performing t
✦ LIBER ✦
An improved method for the isolation of total RNA from spurce tissues
✍ Scribed by Mouna Bahloul; Gérard Burkard
- Book ID
- 112821714
- Publisher
- Springer
- Year
- 1993
- Tongue
- English
- Weight
- 186 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0735-9640
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We have developed a simple and rapid procedure for the isolation of total RNA from small amounts of adipose tissue. Using this method, it is possible to obtain quantitative recovery of RNA from less than 300 mg of adipose tissue, with an average yield of 70 micrograms of RNA per gram of adipose tiss