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An Improved CTAB–Ammonium Acetate Method for Total RNA Isolation from Cotton

✍ Scribed by Lu Zhao; Qi Ding; Jun Zeng; Fu-Rong Wang; Jun Zhang; Shou-Jin Fan; Xin-Qiang He

Book ID
112168343
Publisher
John Wiley and Sons
Year
2012
Tongue
English
Weight
109 KB
Volume
23
Category
Article
ISSN
0958-0344

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In the present study, we describe a method that we developed to isolate total RNA from porcine adipose tissue. This method entails homogenizing porcine adipose tissue in 10 ml of 4 M guanidium thiocyanate, 25 mM sodium citrate, 0.5% Sarcosyl, 0.1 M beta-mercaptoethanol, pH 7.0, and then performing t