An improved method for the determination of 5-phosphoribosyl 1-pyrophosphate
โ Scribed by V. Micheli; G. Pompucci; R. Marcolongo
- Book ID
- 115820657
- Publisher
- Elsevier Science
- Year
- 1975
- Tongue
- English
- Weight
- 358 KB
- Volume
- 65
- Category
- Article
- ISSN
- 0009-8981
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
A method for the measurement of erythrocyte 5-phosphoribosyl 1-pyrophosphate (PP-ribose-P) using HPLC is described. lnosinic acid formed from the enzyme-catalyzed reaction of hypoxanthine and PP-ribose-P using partially purified hypoxanthine-guanine phosphoribosyltransferase is measured after chroma
The properties of an assay for the 5-phosphoribosyl-1-pyrophosphate (PRPP) content of cultured mammalian fibroblasts are described. The assay is based upon the PRPP-dependent release of "CO, from [carboxy/-"Clorotic acid by a commercially available preparation of yeast orotidine-5'-monophosphate pyr