A spectrophotometric method for the determination of 5-phosphoribosyl-1-pyrophosphate
โ Scribed by C. Salerno; A. Giacomello; E. Messina
- Book ID
- 112658738
- Publisher
- Springer
- Year
- 1979
- Tongue
- English
- Weight
- 218 KB
- Volume
- 35
- Category
- Article
- ISSN
- 1420-682X
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๐ SIMILAR VOLUMES
A method for the measurement of erythrocyte 5-phosphoribosyl 1-pyrophosphate (PP-ribose-P) using HPLC is described. lnosinic acid formed from the enzyme-catalyzed reaction of hypoxanthine and PP-ribose-P using partially purified hypoxanthine-guanine phosphoribosyltransferase is measured after chroma
The properties of an assay for the 5-phosphoribosyl-1-pyrophosphate (PRPP) content of cultured mammalian fibroblasts are described. The assay is based upon the PRPP-dependent release of "CO, from [carboxy/-"Clorotic acid by a commercially available preparation of yeast orotidine-5'-monophosphate pyr