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Determination of 5-phosphoribosyl 1-pyrophosphate in mouse liver

โœ Scribed by Marcel Lalanne; J.Frank Henderson

Book ID
107711615
Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
710 KB
Volume
62
Category
Article
ISSN
0003-2697

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โœ S.Randolph May; Robert S. Krooth ๐Ÿ“‚ Article ๐Ÿ“… 1976 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 833 KB

The properties of an assay for the 5-phosphoribosyl-1-pyrophosphate (PRPP) content of cultured mammalian fibroblasts are described. The assay is based upon the PRPP-dependent release of "CO, from [carboxy/-"Clorotic acid by a commercially available preparation of yeast orotidine-5'-monophosphate pyr