A sensitive method for estimating 5-phosphoribosyl 1-pyrophosphate in Escherichia coli
โ Scribed by Aldo S. Bagnara; Alana Mitchell; Iris L. Sin; Lloyd R. Finch
- Book ID
- 107711469
- Publisher
- Elsevier Science
- Year
- 1973
- Tongue
- English
- Weight
- 581 KB
- Volume
- 54
- Category
- Article
- ISSN
- 0003-2697
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A method for the measurement of erythrocyte 5-phosphoribosyl 1-pyrophosphate (PP-ribose-P) using HPLC is described. lnosinic acid formed from the enzyme-catalyzed reaction of hypoxanthine and PP-ribose-P using partially purified hypoxanthine-guanine phosphoribosyltransferase is measured after chroma
The nucleotide sequence of the rpsE gene for the ribosomal protein S5 of a mutant of E. coli showing cold-sensitive growth revealed the presence of an insertion sequence, IS1, near the 3'-end of the gene. This mutant grows very slowly even at the "permissive temperature" (30 degrees C). At temperatu