✦ LIBER ✦

Intracellular concentration of inorganic pyrophosphate in the cells of Escherichia coli: A method for its determination

✍ Scribed by Jukka Heinonen

Book ID: 107711588
Publisher: Elsevier Science
Year: 1974
Tongue: English
Weight: 596 KB
Volume: 59
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(74)90288-7

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

A method for the concentration and for t

A method for the concentration and for the colorimetric determination of nanomoles of inorganic pyrophosphate

✍ Jukka K. Heinonen; Sirpa H. Honkasalo; Eila I. Kukko 📂 Article 📅 1981 🏛 Elsevier Science 🌐 English ⚖ 689 KB

A generally applicable, inexpensive, and sensitive method for the determination of inorganic pyrophosphate (PPJ was developed. PPi was quantitatively separable from solution even in nanomolar concentrations by filtration through a membrane filter in the presence of CaCl\* and KF. The separated #'Pi

A new method for the accurate and rapid

A new method for the accurate and rapid determination of the concentrations of intracellular metabolites in cells during fermentation

✍ Ayaaki Ishizaki; Koh Yamamoto; Yoshifumi Furuta 📂 Article 📅 1995 🏛 Springer-Verlag 🌐 English ⚖ 305 KB

Effect of dissolved O concentration on t

Effect of dissolved O concentration on the Escherichia coli in a cell-recycle bioreactor

✍ I-Ming Chu; Hong-Wei Yen 📂 Article 📅 1997 🏛 Springer Netherlands 🌐 English ⚖ 245 KB

A method for determination of the synthe

A method for determination of the synthesis rate of stable and unstable ribonucleic acid in Escherichia coli

✍ Patrick P. Dennis; Hans Bremer 📂 Article 📅 1973 🏛 Elsevier Science 🌐 English ⚖ 748 KB

A method to determine the relative rates of stable and unstable RNA synthesis is presented. The method employs the antibiotic rifampicin. For exponential phase Escherichia coli B/r growing at rates between 0.67 and 2.10 doublings per hr (3.2-fold range in growth rate), the fraction of the instantane

A continuous assay for an intracellular

A continuous assay for an intracellular enzyme: The analysis of acetate kinase in Escherichia coli

✍ H.Michael Koplove; Charles L. Cooney 📂 Article 📅 1976 🏛 Elsevier Science 🌐 English ⚖ 452 KB

A continuous, automated assay for the measurement of acetate kinase activity in Escherichia co/i is described. The assay system consists of three steps: continuous sonication, acetate kinase catalyzed production of ATP, and subsequent measurement of ATP concentration. Complete extraction of acetate

A method for the determination of 5-phos

A method for the determination of 5-phosphoribosyl 1-pyrophosphate concentrations in erythrocytes using high-performance liquid chromatography

✍ Harry J. Rylance; Ruth C. Wallace; George Nuki 📂 Article 📅 1987 🏛 Elsevier Science 🌐 English ⚖ 416 KB

A method for the measurement of erythrocyte 5-phosphoribosyl 1-pyrophosphate (PP-ribose-P) using HPLC is described. lnosinic acid formed from the enzyme-catalyzed reaction of hypoxanthine and PP-ribose-P using partially purified hypoxanthine-guanine phosphoribosyltransferase is measured after chroma