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An enzyme-linked immunosorbent assay for detection of antibodies against halothane-altered hepatocyte antigens

โœ Scribed by J.G. Kenna; J. Neuberger; Roger Williams


Book ID
118899448
Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
593 KB
Volume
75
Category
Article
ISSN
0022-1759

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An Enzyme-Linked Immunosorbant Assay (EL
โœ Marshall M. Kaplan; John V. Gandolfo; Elaine G. Quaroni ๐Ÿ“‚ Article ๐Ÿ“… 1984 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 457 KB

W e have developed an enzyme linked immunosorbant assay (ELISA) for antimitochondrial antibody. Polyvinyl microtiter plate wells are coated with partially purified rat kidney mitochondria, and excess protein binding sites are blocked with bovine serum albumin. Human serum, diluted 1: 1,000, is incub