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An enzyme-linked immunosorbent assay for the detection of hepatocyte plasma membrane antibodies

✍ Scribed by N.R. Swanson; W.N. Bartholomeus; W.D. Reed; R.A. Joske

Book ID
118899930
Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
884 KB
Volume
85
Category
Article
ISSN
0022-1759

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✍ Marshall M. Kaplan; John V. Gandolfo; Elaine G. Quaroni πŸ“‚ Article πŸ“… 1984 πŸ› John Wiley and Sons 🌐 English βš– 457 KB

W e have developed an enzyme linked immunosorbant assay (ELISA) for antimitochondrial antibody. Polyvinyl microtiter plate wells are coated with partially purified rat kidney mitochondria, and excess protein binding sites are blocked with bovine serum albumin. Human serum, diluted 1: 1,000, is incub