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An enzyme-linked immunosorbent assay for detection of flavivirus antibodies in chicken sera

✍ Scribed by A.K. Broom; J. Charlick; S.J. Richards; J.S. Mackenzie

Book ID
119114441
Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
603 KB
Volume
15
Category
Article
ISSN
0166-0934

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✍ Marshall M. Kaplan; John V. Gandolfo; Elaine G. Quaroni πŸ“‚ Article πŸ“… 1984 πŸ› John Wiley and Sons 🌐 English βš– 457 KB

W e have developed an enzyme linked immunosorbant assay (ELISA) for antimitochondrial antibody. Polyvinyl microtiter plate wells are coated with partially purified rat kidney mitochondria, and excess protein binding sites are blocked with bovine serum albumin. Human serum, diluted 1: 1,000, is incub