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An Enzyme-Linked Immunosorbent Assay for Detecting Antibodies to Pasteurella multocida in Chickens

โœ Scribed by D. J. Briggs and J. K. Skeeles

Book ID
118125396
Publisher
Bioone
Year
1984
Tongue
English
Weight
746 KB
Volume
28
Category
Article
ISSN
0005-2086

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โœ Marshall M. Kaplan; John V. Gandolfo; Elaine G. Quaroni ๐Ÿ“‚ Article ๐Ÿ“… 1984 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 457 KB

W e have developed an enzyme linked immunosorbant assay (ELISA) for antimitochondrial antibody. Polyvinyl microtiter plate wells are coated with partially purified rat kidney mitochondria, and excess protein binding sites are blocked with bovine serum albumin. Human serum, diluted 1: 1,000, is incub