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The enzyme-linked immunosorbent assay for detecting antibodies against Dirofilaria immitis in dogs

✍ Scribed by K. Matsumura; Y. Kazuta; R. Endo; K. Tanaka; T. Inoue

Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
430 KB
Volume
21
Category
Article
ISSN
0304-4017

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✍ Marshall M. Kaplan; John V. Gandolfo; Elaine G. Quaroni πŸ“‚ Article πŸ“… 1984 πŸ› John Wiley and Sons 🌐 English βš– 457 KB

W e have developed an enzyme linked immunosorbant assay (ELISA) for antimitochondrial antibody. Polyvinyl microtiter plate wells are coated with partially purified rat kidney mitochondria, and excess protein binding sites are blocked with bovine serum albumin. Human serum, diluted 1: 1,000, is incub