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Enzyme linked immunosorbent assay for detection of antibodies againstBesnoitia besnoitiin cattle

✍ Scribed by Varda Shkap; Hanna Ungar-Waron; E. Pipano; C. Greenblatt


Publisher
Springer Netherlands
Year
1984
Tongue
English
Weight
478 KB
Volume
16
Category
Article
ISSN
0049-4747

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An Enzyme-Linked Immunosorbant Assay (EL
✍ Marshall M. Kaplan; John V. Gandolfo; Elaine G. Quaroni πŸ“‚ Article πŸ“… 1984 πŸ› John Wiley and Sons 🌐 English βš– 457 KB

W e have developed an enzyme linked immunosorbant assay (ELISA) for antimitochondrial antibody. Polyvinyl microtiter plate wells are coated with partially purified rat kidney mitochondria, and excess protein binding sites are blocked with bovine serum albumin. Human serum, diluted 1: 1,000, is incub