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Use of the enzyme-linked immunosorbent assay for detection of antibodies toChlamydia trachomatis

✍ Scribed by Duc-Goiran, P. ;Raymond, J. ;Léauté, J. B. ;Orfila, J.

Publisher
Springer-Verlag
Year
1983
Weight
509 KB
Volume
2
Category
Article
ISSN
0722-2211

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✍ Marshall M. Kaplan; John V. Gandolfo; Elaine G. Quaroni 📂 Article 📅 1984 🏛 John Wiley and Sons 🌐 English ⚖ 457 KB

W e have developed an enzyme linked immunosorbant assay (ELISA) for antimitochondrial antibody. Polyvinyl microtiter plate wells are coated with partially purified rat kidney mitochondria, and excess protein binding sites are blocked with bovine serum albumin. Human serum, diluted 1: 1,000, is incub