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Enzyme-linked immunosorbent assays for sperm antibody detection and antigenic analysis

✍ Scribed by S. Paul; V. Baukloh; L. Mettler

Book ID
118899208
Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
291 KB
Volume
56
Category
Article
ISSN
0022-1759

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✍ Marshall M. Kaplan; John V. Gandolfo; Elaine G. Quaroni πŸ“‚ Article πŸ“… 1984 πŸ› John Wiley and Sons 🌐 English βš– 457 KB

W e have developed an enzyme linked immunosorbant assay (ELISA) for antimitochondrial antibody. Polyvinyl microtiter plate wells are coated with partially purified rat kidney mitochondria, and excess protein binding sites are blocked with bovine serum albumin. Human serum, diluted 1: 1,000, is incub