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An enzyme-linked immunosorbent assay for antibody against acetylcholine receptor

โœ Scribed by Channing L. Hinman; Richard A. Hudson; C. Lynne Burek; Gerald Goodlow; Helene C. Rauch


Book ID
107903100
Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
718 KB
Volume
9
Category
Article
ISSN
0165-0270

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An Enzyme-Linked Immunosorbant Assay (EL
โœ Marshall M. Kaplan; John V. Gandolfo; Elaine G. Quaroni ๐Ÿ“‚ Article ๐Ÿ“… 1984 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 457 KB

W e have developed an enzyme linked immunosorbant assay (ELISA) for antimitochondrial antibody. Polyvinyl microtiter plate wells are coated with partially purified rat kidney mitochondria, and excess protein binding sites are blocked with bovine serum albumin. Human serum, diluted 1: 1,000, is incub