An enzyme linked immunosorbent assay for leucocyte and platelet antibodies
β Scribed by Robert Doughty; Virge James; Jill Magee
- Book ID
- 118898257
- Publisher
- Elsevier Science
- Year
- 1981
- Tongue
- English
- Weight
- 456 KB
- Volume
- 47
- Category
- Article
- ISSN
- 0022-1759
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W e have developed an enzyme linked immunosorbant assay (ELISA) for antimitochondrial antibody. Polyvinyl microtiter plate wells are coated with partially purified rat kidney mitochondria, and excess protein binding sites are blocked with bovine serum albumin. Human serum, diluted 1: 1,000, is incub