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An Enzyme-Linked Immunosorbent Assay (ELISA) for Detecting Chicken Anti-Reovirus Antibody at High Sensitivity

✍ Scribed by S. S. Slaght, T. J. Yang, L. van der Heide and T. N. Fredrickson


Book ID
124765043
Publisher
Bioone
Year
1978
Tongue
English
Weight
425 KB
Volume
22
Category
Article
ISSN
0005-2086

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✍ Marshall M. Kaplan; John V. Gandolfo; Elaine G. Quaroni πŸ“‚ Article πŸ“… 1984 πŸ› John Wiley and Sons 🌐 English βš– 457 KB

W e have developed an enzyme linked immunosorbant assay (ELISA) for antimitochondrial antibody. Polyvinyl microtiter plate wells are coated with partially purified rat kidney mitochondria, and excess protein binding sites are blocked with bovine serum albumin. Human serum, diluted 1: 1,000, is incub