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An Enzyme Linked Immunosorbent Assay (ELISA) for Detecting IgG Sensitized Erythrocytes

✍ Scribed by K. W. Bruner Jr.; C. W. Kissling

Book ID
114437401
Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
316 KB
Volume
19
Category
Article
ISSN
0372-1248

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An Enzyme-Linked Immunosorbant Assay (ELISA) for Detecting Antimitochondrial Antibody
✍ Marshall M. Kaplan; John V. Gandolfo; Elaine G. Quaroni πŸ“‚ Article πŸ“… 1984 πŸ› John Wiley and Sons 🌐 English βš– 457 KB

W e have developed an enzyme linked immunosorbant assay (ELISA) for antimitochondrial antibody. Polyvinyl microtiter plate wells are coated with partially purified rat kidney mitochondria, and excess protein binding sites are blocked with bovine serum albumin. Human serum, diluted 1: 1,000, is incub