An enzymatic method for the determination of hemoglobinA1C
β Scribed by Kozo Hirokawa; Kazuhiko Shimoji; Naoki Kajiyama
- Book ID
- 106363438
- Publisher
- Springer Netherlands
- Year
- 2005
- Tongue
- English
- Weight
- 186 KB
- Volume
- 27
- Category
- Article
- ISSN
- 0141-5492
No coin nor oath required. For personal study only.
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## B i o l o g i c a l l y l a b e l l e d [l-14C) g l y c e r o l ( L -[ l -' " C ) g l y c e r o l ) 048 prepared by a two s t e p p r o c e s s . S t e p one i n v o 1 v e d t h e p r e p a r a t i o n of [I -C ] g 2 y ce r o 1 3 -p ho sp ha t e from l4C02 and D-ribuloee 1,5 -diphoephate u s i
An enzymatic method for the determination of picomol amounts of dCMP in cellfree extracts was developed. The method is based on the quantitative phosphorylation of dCMP to dCTP by a partially purified dCMP-kinase and a pure NDP-kinase. The dCTP formed is measured using a DNApolymerase assay. With t