An enzymatic method for [32P]phosphoenolpyruvate synthesis
β Scribed by Roshan L. Mattoo; E.Bruce Waygood
- Publisher
- Elsevier Science
- Year
- 1983
- Tongue
- English
- Weight
- 382 KB
- Volume
- 128
- Category
- Article
- ISSN
- 0003-2697
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π SIMILAR VOLUMES
An enzymatic method for the synthesis of [beta-32P]ADP from [gamma-32P]ATP is described. This substrate is required for the assay of ADPase and is not commercially available. The method described results in a preparation of [beta-32P]ADP of high purity with a yield of approximately 40% the theoretic
A simple, rapid, and inexpensive method is described for the synthesis of gamma-32P-labeled ribo- or deoxyribonucleoside triphosphates. The procedure involves chemical synthesis of [32P]acetylphosphate and subsequent phosphorylation of nucleoside diphosphates using acetate kinase (EC 2.7.2.1) and a