Three maltases have been detected in each of a variety of strains of Saccharomyces. These are present in constant relative amounts in a particular strain and are characterized by reproducible first order thermal decay constants. Four of six mutants, temperature-sensitive for maltose fermentation, la
An enrichment method for temperature-sensitive and auxotrophic mutants of yeast
β Scribed by Walton, E. Fintan ;Carter, Bruce L. A. ;Pringle, John R.
- Publisher
- Springer
- Year
- 1979
- Tongue
- English
- Weight
- 313 KB
- Volume
- 171
- Category
- Article
- ISSN
- 0026-8925
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An E. coli strain deleted in the region malasd is used for the selection of conditional or auxotrophic mutants. Thermosensitive and auxotrophic strains have thus been isolated on plates. After selection in liquid medium, a strain has been isolated which is sensitive to excess one-carbon metabolites.
## Abstract Mammalian cell populations may be enriched for temperatureβsensitive (__ts__) mutants by the tritiated thymidine (^3^HβTdR) suicide procedure (Thompson et al., '71). Such procedures were carried out on the nearβdiploid Chinese hamster ovary (CHO) cell line and on a line made βtetraploid