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An enrichment method for temperature-sensitive and auxotrophic mutants of yeast

✍ Scribed by Walton, E. Fintan ;Carter, Bruce L. A. ;Pringle, John R.


Publisher
Springer
Year
1979
Tongue
English
Weight
313 KB
Volume
171
Category
Article
ISSN
0026-8925

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## Abstract Mammalian cell populations may be enriched for temperature‐sensitive (__ts__) mutants by the tritiated thymidine (^3^H‐TdR) suicide procedure (Thompson et al., '71). Such procedures were carried out on the near‐diploid Chinese hamster ovary (CHO) cell line and on a line made β€œtetraploid