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Thermal inactivation of maltase and its application to temperature-sensitive mutants of yeast

✍ Scribed by Eaton, Norman R. ;Zimmermann, F. K.


Publisher
Springer
Year
1976
Tongue
English
Weight
444 KB
Volume
148
Category
Article
ISSN
0026-8925

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✦ Synopsis


Three maltases have been detected in each of a variety of strains of Saccharomyces. These are present in constant relative amounts in a particular strain and are characterized by reproducible first order thermal decay constants. Four of six mutants, temperature-sensitive for maltose fermentation, lack the maltase of intermediate stability. This deficiency appears to be the basis of the temperature sensitivity, and it is concluded that these strains carry a mutation in the structural gene for that maltase.


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