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An efficient one-step site-directed and site-saturation mutagenesis protocol

✍ Scribed by Zheng, L.


Book ID
118252433
Publisher
Oxford University Press
Year
2004
Tongue
English
Weight
175 KB
Volume
32
Category
Article
ISSN
0305-1048

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One-Step site-directed mutagenesis of AT
✍ Shaun P. Scott; Alison Teh; Cheng Peng; Martin F. Lavin πŸ“‚ Article πŸ“… 2002 πŸ› John Wiley and Sons 🌐 English βš– 28 KB πŸ‘ 1 views

In vitro mutagenesis of large genes has proven to be difficult for a number of reasons, including the number of steps involved and the instability of large inserts. We describe here a single-step PCR method to introduce mutations into an ataxia-telangiectasia mutated (ATM) gene cDNA construct (20 kb