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One-step site-directed mutagenesis of viroid dimeric cDNA

✍ Scribed by Rafael Sanjuán; José-Antonio Daròs

Book ID
113767594
Publisher
Elsevier Science
Year
2007
Tongue
English
Weight
263 KB
Volume
145
Category
Article
ISSN
0166-0934

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✍ Shaun P. Scott; Alison Teh; Cheng Peng; Martin F. Lavin 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 28 KB 👁 1 views

In vitro mutagenesis of large genes has proven to be difficult for a number of reasons, including the number of steps involved and the instability of large inserts. We describe here a single-step PCR method to introduce mutations into an ataxia-telangiectasia mutated (ATM) gene cDNA construct (20 kb