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An efficient approach for site-directed mutagenesis using central overlapping primers

✍ Scribed by Huiping Wang; Nan Zhou; Feng Ding; Zhaofang Li; Ruichuan Chen; Aidong Han; Runzhong Liu

Book ID
113412169
Publisher
Elsevier Science
Year
2011
Tongue
English
Weight
250 KB
Volume
418
Category
Article
ISSN
0003-2697

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We present a rapid, cheap and highly efficient method for site-directed mutagenesis using the polymerase chain reaction (PCR). This method is applicable to every DNA fragment which has to be cloned into the multiple cloning site of any vector, or vector pair, in two different orientations. It requir