๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

An automated column chromatographic procedure for the fluorometric determination of mixtures of hexoses

โœ Scribed by Peter Weber; Irene Bornstein; Richard J. Winzler

Publisher
Elsevier Science
Year
1966
Tongue
English
Weight
321 KB
Volume
14
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

โœฆ Synopsis

The quantitative determination of individual hexoses in mixtures is of special importance for the elucidation of the structure of the oligosaccharide units of glycoproteins. Because of its high sensitivity we have been able to adapt the fluorometric procedure for glucose (l-3) to the continuous monitoring of effluents from Dowex 1 borate columns (4-6).

The method has been applied to hydrolyzates of a glycopeptide from orosomucoid containing galactose and mannose. The procedure as presently employed is completed in 4.5 hr and requires about 20 pg of each hexose.

๐Ÿ“œ SIMILAR VOLUMES

Modified procedures for the separation a
Modified procedures for the separation and fluorometric determination of adenine nucleotides
โœ Richard B. Triplett; Larry D. Smith ๐Ÿ“‚ Article ๐Ÿ“… 1977 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 334 KB

Modified procedures for separating and determining adenine nucleotides are described. Separation was achieved by one-dimensional thin-layer chromatography on polyethyleneimine-cellulose plates in 1.4 M LiCl after prewashing the spotted plate in deionized water. The fluorometric procedure for determi

An automated method for the determinatio
An automated method for the determination of hexosamines
โœ Endre A. Balazs; Kjell O. Berntsen; Judith Karossa; David A. Swann ๐Ÿ“‚ Article ๐Ÿ“… 1965 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 313 KB

The first practical method for the determination of hexosamines was worked out by Elson and Morgan (1). Since that time many modifications have been introduced in an attempt to increase the sensitivity and reproducibility of the technique (2) and to decrease the effect of interfering substances (3)