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Modified procedures for the separation and fluorometric determination of adenine nucleotides

โœ Scribed by Richard B. Triplett; Larry D. Smith

Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
334 KB
Volume
80
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

Modified procedures for separating and determining adenine nucleotides are described. Separation was achieved by one-dimensional thin-layer chromatography on polyethyleneimine-cellulose plates in 1.4 M LiCl after prewashing the spotted plate in deionized water. The fluorometric procedure for determining adenine and adenine derivatives using glyoxal dihydrate trimer was modified to reduce the heat step from 3 hr to 25 min without loss of sensitivity or stability. These modified procedures were used to determine the adenine nucleotide levels in rat liver.

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