๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

An assay to determine the kinetics of RNA cleavage

โœ Scribed by Robert Corcoran; Marc Labelle; Anthony W. Czarnik; Ronald Breslow

Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
530 KB
Volume
144
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

โœฆ Synopsis

To evaluate some synthetic catalysts that mimic ribonuclease, a quantitative assay has been developed that measures the number of phosphate diester bonds cleaved in a polymeric RNA substrate. This assay involves determining the number of 5'-oligonucleotide termini produced during the cleavage, using polyuridylic acid as the substrate. Samples withdrawn from the kinetic run are treated with venom exonuclease (phosphodiesterase I), and the increase in the concentration of uridine is determined by high-performance liquid chromatography. A related assay has been developed to monitor the catalyzed cleavage of the dinucleotide uridylyl(3'----5') uridine (UpU).

๐Ÿ“œ SIMILAR VOLUMES

An assay for the endonucleolytic cleavag
An assay for the endonucleolytic cleavage of RNA to large oligonucleotides
โœ M.V. Berridge; A.I. Aronson ๐Ÿ“‚ Article ๐Ÿ“… 1973 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 596 KB

A rapid assay for endonuclease activity which cleaves high-molecularweight RNA to acid-precipitable fragments has been developed. RNA is covalently coupled to beaded agarose under conditions that produce relatively few coupling sites. The immobilized RNA can be used qualitatively or semiquantitative

An examination of the rate assay to dete
An examination of the rate assay to determine the active site normality of papain
โœ Bernard R. Glick; Lewis J. Brubacher ๐Ÿ“‚ Article ๐Ÿ“… 1976 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 240 KB

Data are presented which demonstrate that the o-N-benzoyk-arginine ethyl ester rate assay procedure, based on a burst titration with N-benzyloxycarbonyl-L-tyrosinep-nitrophenyl ester as previously described (1). is an accurate and reliable method for determining the normality of papain in solution.