To evaluate some synthetic catalysts that mimic ribonuclease, a quantitative assay has been developed that measures the number of phosphate diester bonds cleaved in a polymeric RNA substrate. This assay involves determining the number of 5'-oligonucleotide termini produced during the cleavage, using
โฆ LIBER โฆ
An examination of the rate assay to determine the active site normality of papain
โ Scribed by Bernard R. Glick; Lewis J. Brubacher
- Publisher
- Elsevier Science
- Year
- 1976
- Tongue
- English
- Weight
- 240 KB
- Volume
- 73
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
โฆ Synopsis
Data are presented which demonstrate that the o-N-benzoyk-arginine ethyl ester rate assay procedure, based on a burst titration with N-benzyloxycarbonyl-L-tyrosinep-nitrophenyl ester as previously described (1). is an accurate and reliable method for determining the normality of papain in solution.
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