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An enzymic method for determination of inorganic pyrophosphate and its use as an assay for RNA polymerase

✍ Scribed by J.C. Johnson; Mike Shanoff; S.T. Bass; J.A. Boezi; R.G. Hansen


Publisher
Elsevier Science
Year
1968
Tongue
English
Weight
524 KB
Volume
26
Category
Article
ISSN
0003-2697

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## Abstract A new type of fluorescence assay for the determination of peroxidase (POx) activity is presented. The assay is based on the indication of the enzymatic consumption of H~2~O~2~ (HP), using a fluorescent europium–tetracycline (Eu~3~TC) complex as indicator. On addition of HP, this complex