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An assay for the endonucleolytic cleavage of RNA to large oligonucleotides

โœ Scribed by M.V. Berridge; A.I. Aronson

Publisher
Elsevier Science
Year
1973
Tongue
English
Weight
596 KB
Volume
53
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A rapid assay for endonuclease activity which cleaves high-molecularweight RNA to acid-precipitable fragments has been developed. RNA is covalently coupled to beaded agarose under conditions that produce relatively few coupling sites. The immobilized RNA can be used qualitatively or semiquantitatively in an assay for endonuclease activity by determining the release of acid-precipitable RNA from the complex. This assay is compared to one employing separation of degraded RNA by gel electrophoresis.

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