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An Approach for Enhancing Heterologous Production of Providencia Rettgeri Penicillin Acylase in Escherichia coli

✍ Scribed by C. Perry Chou; Wen-Chang Wang; Ming-I Lin

Book ID
109386087
Publisher
American Institute of Chemical Engineers
Year
2000
Tongue
English
Weight
95 KB
Volume
16
Category
Article
ISSN
8756-7938

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## Abstract High‐level expression of recombinant penicillin acylase (PAC) using the strong __trc__ promoter system in __Escherichia coli__ is frequently limited by the processing and folding of PAC precursors (proPAC) in the periplasm, resulting in physiological stress and inclusion body formation