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Arabinose-Induction of lac-Derived Promoter Systems for Penicillin Acylase Production in Escherichia coli

✍ Scribed by Niju Narayanan; Ming-Yi Hsieh; Yali Xu; C. Perry Chou

Book ID
109387355
Publisher
American Institute of Chemical Engineers
Year
2006
Tongue
English
Weight
232 KB
Volume
22
Category
Article
ISSN
8756-7938

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## Abstract High‐level expression of recombinant penicillin acylase (PAC) using the strong __trc__ promoter system in __Escherichia coli__ is frequently limited by the processing and folding of PAC precursors (proPAC) in the periplasm, resulting in physiological stress and inclusion body formation